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. 2010 Nov 22;7:335. doi: 10.1186/1743-422X-7-335

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Copyright ©2010 Wu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Suppression of local GFP silencing by RRSV Pns6. (A-F) N. benthamiana line 16c plants were coinfiltrated with Agrobacterium spp. (Agro.) mixtures carrying 35S-GFP and the individual constructs indicated in each image. GFP fluorescence was viewed under longwavelength UV light at 7 days postinfiltration (dpi). (G) Northern blot analysis of the steady-state levels of GFP mRNA and siRNA extracted from different infiltrated patches shown in panel A to F. 28 S rRNA and t RNA were used as loading controls for detection of GFP mRNA and GFP siRNA respectively.