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. 2010 Nov 22;7:335. doi: 10.1186/1743-422X-7-335

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Copyright ©2010 Wu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RRSV Pns6 can not inhibit local silencing induced by dsRNA (A-C) N. benthamiana line 16c plants were coinfiltrated with Agrobacterium spp. (Agro.) mixtures carrying 35S-dsGFP and the individual constructs indicated in each image. GFP fluorescence was viewed under longwavelength UV light at 7 days postinfiltration (dpi). (D) Northern blot analysis of the steady-state levels of GFP mRNA extracted from different infiltrated patches shown in panel A to C. The bottom gel shows rRNA with ethidium bromide staining as a loading control.