Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 7;7:59. doi: 10.1186/1476-9255-7-59

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2010 Oyegunwa et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

TMP inhibits TNF-α and MCP-1/CCL-2 protein and mRNA. RAW 264.7 cells were either stimulated with 1 μg/ml of LPS or 1 μg/ml of LPS and 25 μM TMP. Following 24 h of treatment, supernatants were collected and levels of TNF-α (A) and MCP-1/CCL2 (B) were determined by ELISA. To assess the effects of TMP on the transcription of TNF-α (C) and MCP-1/CCL2 (D) genes, RNA was prepared from RAW 264.7 cells stimulated with 1 μg/ml of LPS or 1 μg/ml of LPS and 25 μM TMP for the indicated time periods. Quantitative RT-PCR was used to analyze the levels of TNF-α and MCP-1/CCL2 mRNA. Asterisks indicate significant differences between treatments with LPS and LPS and TMP (p < 0.05, T-test).