Table 2.
Composition of growth medium and production medium used for arnebia cell suspension culture.
| S. No. | Constituents | Concentration (mgl-1) | |
|---|---|---|---|
| Growth medium | Production medium (M9) | ||
| 1. | CaCl2.2H2O | 440.0 | -- |
| 2. | Ca(NO3)2.7H2O | -- | 694.0 |
| 3. | NH4NO3 | 1650.0 | -- |
| 4. | MgSO4.7H2O | 370.0 | 750.0 |
| 5. | KH2PO4 | 170.0 | -- |
| 6. | KNO3 | 1900 | 80.0 |
| 7. | Na2EDTA.2H2O | 37.2 | 19.0 |
| 8. | Na2SO4 | -- | 1480.0 |
| 9. | FeSO4.7H2O | 27.8 | 27.8 |
| 10. | Na2MoO4.2H2O | 0.25 | -- |
| 11. | H3BO3 | 6.2 | 4.5 |
| 12. | CoCl2.6H2O | 0.025 | -- |
| 13. | CuSO4.5H2O | 0.025 | 0.3 |
| 14. | ZnSO4. 7H2O | 8.6 | 3.0 |
| 15. | KCl | -- | 65.0 |
| 16 | MnSO4.H2O | 16.9 | -- |
| 17. | KI | 0.83 | -- |
| 18. | Mesoinositol | 100 | -- |
| 19. | Glycine | 2.0 | -- |
| 20. | Nicotinic acid | 0.5 | -- |
| 21. | Pyridoxine HCl | 0.5 | -- |
| 22. | Thiamine HCl | 0.11 | -- |
| 23 | Sucrose | 30000 | 30000 |
| 24. | 6-Benzyl amino-purine (BAP) | 10.0 μM | -- |
| 25 | Indole-3-butyric acid (IBA) | 5.0 μM | -- |
Growth medium is referred to as low shikonins production system (LSPS), whereas production medium constitutes high shikonins production system (HSPS)