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. 2010 Dec 15;21(24):4312–4324. doi: 10.1091/mbc.E10-04-0310

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 1. — The expression of RhoS/RSA-14-44 is restricted to germ cells. (A) Tissue distribution of RhoS/RSA-14-44 mRNA in adult rats was analyzed by Northern blot. Each lane contains 20 μg of total RNA. (B) The spermatogenic cell–specific expression pattern of RhoS. Immunohistochemistry was carried out using anti-RhoS polyclonal antibodies (right) to clarify the localization of the protein in rat testis. A negative control was performed using normal rabbit sera (left). P, pachytene spermatocytes; Rs, round spermatids; Es, elongated spermatids; L, lumen of seminiferous. Bar, 100 μm in top panels and 20 μm in bottom panels.