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Transient expression of RhoS has little effect on the cellular proteasome. HEK293T cells were transfected with indicated plasmids. The Western blot analysis of target proteins, coupled with a cellular proteasome activity assay, were performed on the cells harvested at different time points, i.e., A and C, 36 h and B and D, 72 h after transfection. Data are presented as the mean ± SD of three independent experiments. P, precursor; M, matured.
