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. 2010 Dec 15;21(24):4312–4324. doi: 10.1091/mbc.E10-04-0310

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 9. — PSMB5 serves as a novel effector of RhoS. (A) GTP loading is not essential for the association between RhoS and PSMB5. The efficiency of RhoS and its mutants in coimmunoprecipitating of PSMB5 precursors were compared to evaluate the effect of GTP binding on the association between RhoS and PSMB5. (B) The activation of RhoS is a prerequisite for its activity in down-regulating the protein stability of PSMB5 precursors. The same group of RhoS mutants used in A was introduced into the protein stability assay based on CHX treatment as described above. Total lysates, 10 μg, were subjected to the Western blot analysis with GFP and GAPDH as transfection efficiency and loading controls. M, MG132.