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. 2010 Dec 15;21(24):4361–4372. doi: 10.1091/mbc.E10-04-0298

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 6. — Centriole overduplication in GFP-Cetn2–expressing cells. (A) Extra Cetn2-containing foci contain several centriole markers. HeLa cells transfected GFP-Cetn2^TTD and arrested in S-phase for 48 h were stained with antibodies against centriolar markers Cep135 and CP110. Shown are whole cell images (bar = 5 μm) and digitally magnified images of centrosomes (bar = 1 μm); green, GFP; red, Cep135 or CP110. (B) Extra Cetn2-containing foci recruit γ-Tubulin and participate in mitotic spindle assembly. HeLa cells were transfected with empty vector or untagged Cetn2 and analyzed with antibodies against Cetn2 and γ-Tubulin. Shown are cells with either pseudobipolar (left panel) or multipolar mitotic spindles from the Cetn2 transfection; green, Cetn2; red, γ-Tubulin. Bar = 5 μm. Insets show unmagnified images of spindle poles.