Figure 5.

zds1Δ zds2Δ bud morphology defect requires CDC55. (A) Deletion of CDC55 in a zds1Δ zds2Δ strain suppressed the mutant, elongated bud phenotype at 30°C but not at 18°C. DIC micrographs of representative congenic, log-phase cdc55Δ (KKY1182), zds1Δ zds2Δ (KKY542), and cdc55Δ zds1Δ zds2Δ (KKY1200) cells cultured at 18 or 30°C in rich medium (YPD) for 4 h, after initial culturing at 30°C. Scale bar, 5 μm. (B) Quantification of mutant bud morphology in cultures of isogenic cdc55Δ zds1Δ zds2Δ strains containing (KKY1201; middle set of columns) or not containing (KKY1200; left set of columns) a CEN plasmid with CDC55. The KKY1201 strain was treated with 5-FOA to select for cells that had lost the CDC55 plasmid (right set of columns). Strains were grown as described in A. Elongated bud morphology was scored by DIC microscopy. n = 200 cells scored for each strain. Error bars, SD across three independent experiments.