Table 2.
Plasmids used in this study
| Plasmid | Relevant genotype | Source |
|---|---|---|
| M2654 | pTF63 (ZDS2) | Yu et al. (1996) |
| M2739 | YEp24 (ZDS1) | Yu et al. (1996) |
| p4339 | TA::MX4-natR switcher cassette | Tong et al. (2001) |
| pDD42 (alias pRB1438) | pRS316 (GAL) | Kozminski et al. (2000) |
| pKK1575 | YCplac111 (ZDS2) | This studya |
| pKK1802 | pAlter1 (zds1::HIS3) | Bi and Pringle (1996) |
| pKK1876 | Ycplac111 (zds2-3R863H, V868A) | This studya |
| pKK1884 | YCplac111 (zds2-i-9xmyc) | This studya |
| pKK1886 | YCplac111 (zds2-3-i-9xmyc) | This studya |
| pKK1916 | YCplac111 (zds2Δ129-479) | This studya |
| pKK1917 | YCplac111 (zds2Δ13-130) | This studya |
| pKK1918 | YCplac111 (zds2Δ808-912) | This studya |
| pKK1919 | YCplac111 (zds2Δ813-942) | This studya |
| pKK1920 | YCplac111 (zds2Δ573-882) | This studya |
| pKK1930 | pRS316 (GAL-ZDS1) | This studya |
| pKK1931 | pRS316 (GAL-ZDS2) | This studya |
| pKK1932 | pRS316 (GAL-HSL7) | This studya |
| pKK1934 | pGEX-4T1 (GST-ZDS2(731-942)) | This studya |
| pKK1938 | YCplac111(ZDS2) | This studya |
| pKK1939 | pOBD2 (GAL4DBD-ZDS2) | This studya |
| pKK1940 | pOBD2 (GAL4DBD-zds2Δ808-912) | This studya |
| pKK1941 | pRS425 (CDC55) | This studya |
| pKK1944 | pMAL-2c (CDC55) | This studya |
| pKK1954 | pRS316 (GAL- zds2Δ808-912) | This studya |
| pGEX-4T1 | (GST) | GE Healthcare |
| pMAL-2c | (MBP) | New England Biolabs |
| pML1 | pFA6a-kanMX6 | Longtine et al. (1998) |
| pOBD2 | CEN TRP1 GAL4-DBD | Uetz et al. (2000) |
| pOM22 | pOM (9xmyc:K. lactis URA3) | Gauss et al. (2005)b |
| pRS425 | 2μ LEU2 | Sikorski and Hieter (1989) |
| pSH65 | pGAL-cre | Gueldener et al. (2002) |
| pCMY14 | pRS424 (CDC55) | D. Burkec |
| pCMY18 | pRS416 (CDC55) | D. Burkec |
| YEplac195 | 2μ URA3 | Gietz and Sugino (1988) |
| YCplac33 | CEN URA3 | Gietz and Sugino (1988) |
| YCplac111 | CEN LEU2 | Gietz and Sugino (1988) |
All plasmids confer ampicillin resistance.
a See Materials and Methods.
b Obtained from EUROSCARF (Frankfurt, Germany).
c Gift of D. Burke (University of Virginia, Charlottesville, VA).