Figure 2.

Expression of wild-type and mutant human dopamine transporter in HEK293 cells

Protein was probed with antibodies against the C-terminal (A) and N-terminal (B) of the dopamine transporter, and with anti-β-actin antibody to show the relative equivalent loading of total protein (C). The same amount of total lysate protein was loaded in all lanes. The mutations not reported in previous studies are shown in the same sequence as in webappendix p 10, starting with patient 5.