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. 2010 Sep 6;588(Pt 21):4145–4163. doi: 10.1113/jphysiol.2010.193268

Figure 8. Dopamine plays a role in the light-induced increase in the coupling of OFF α-GCs.

Figure 8

A, tracer coupling pattern seen in the dark-adapted rabbit retina after injection of an OFF α-GC (asterisk) with Neurobiotin and application of the NO donor SNAP (500 μm). There was no significant change in the coupling pattern relative to that seen in dark-adapted retinas under control conditions. B, tracer coupling pattern seen in the dark-adapted rabbit retina after application of the dopamine D1 receptor agonist SKF-38393 (100 μm). There was no significant difference in the coupling pattern relative to that seen in dark-adapted retinas under control conditions. C, tracer coupling pattern seen in the dark-adapted rabbit retina after injection of an OFF α-GC (asterisk) with Neurobiotin and application of the dopamine D2 receptor blocker eticlopride (25 μm). There was significant increase in the intensity, number, and extent of coupled amacrine and ganglion cells. D, tracer coupling pattern seen in the dark-adapted rabbit retina after injection of an OFF α-GC (asterisk) with Neurobiotin and application of the dopamine D2 receptor antagonist spiperone (10 μm). There was significant increase in the intensity, number and extent of coupled amacrine and ganglion cells. E, bar graph comparing the number of coupled α-GCs under different adaptational conditions and following the application of dopaminergic agents. The asterisks indicate a statistically significant change from values measured under dark-adapted, control conditions. MDL, MDL-12330; Rp-8-CPT-cA, Rp-8-CPT-cAMPS. Number of cells for each condition: Dark = 10; Log −3.5 = 10; Log 0.0 = 10; Dark + D2 Blocker = 10; Log 0.0 + D2 Blocker = 10; Log 0.0 + MDL = 5; Log 0.0 + Rp-8-CPT-cA = 5. F, bar graph comparing the number of coupled amacrine cells under different adaptational conditions and following the application of dopaminergic agents. Number of cells is the same as in E. G, bar graph comparing the number of coupled α-GCs under different adaptation conditions and following the application of dopaminergic agents. Number of cells is the same as in E.