Figure 5. Expression of phosphorylated NR1 (pNR1) and total NR1 in spinal cord dorsal horn extracts from non-diabetic (Non-D), non-supplemented STZ-diabetic (Non-suppl. STZ-D) and MgSO₄-supplemented (MgSO₄-suppl. STZ-D) rats at week 4 of the experiment.

A, representative Western blots showing pNR1 (upper panel) or total NR1 (lower panel) expression; B and C, percentage changes of pNR1 and NR1 levels. Data are means ±s.e.m. expressed as a percentage of values measured in Non-D rats (% of controls), for 3 Non-D rats, 3 MgSO₄-suppl. STZ-D rats and 3 Non-suppl. STZ-D rats. Statistical analysis was performed by one-way ANOVA followed by a Tukey–Kramer test. ***P < 0.001 vs. N rats; ##P < 0.01 vs. Non-suppl. STZ-D rats.