Fig. 1.
Effect of oxidative stress on photosynthetic pigments in TR1 R. farinacea phycobiont. Liquid cultures of isolated TR1 algae were subjected to oxidative stress through incubations with the ROS propagator CuHP at the indicated concentrations. At the indicated times, carotenoids and chloroplylls a and b were determined spectrophotometrically as described in Materials and Methods. Initial (incubation time = 0) values of chlorophyll a, chlorophyll b and carotenoids were 1·98 (± 0·10), 0·35 (± 0·013) and 0·86 (± 0·039) μg per 106 cells, respectively. Values represent mean results of 4–5 independent experiments; s.d. <5 % in all cases.