Fig. 3.

Changes in PSI, PSII and HSP70 proteins in TR1 and TR9 R. farinacea phycobionts exposed to oxidative stress. Liquid cultures of isolated TR1 or TR9 algae were subjected to oxidative stress through incubations with the ROS propagator CuHP at the indicated concentrations. At the indicated times, soluble and membrane proteins were extracted and separated by differential centrifugation as described in Materials and methods. PSI (PsaC) and PSII (D1) proteins encoded by psaC and psbA genes, respectively, were immunodetected by Western blotting of the membrane protein fraction and HSP70 by Western blotting of the soluble protein fraction.