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. 2010 Nov 1;69(11):2042–2050. doi: 10.1136/ard.2009.126383

Figure 2.

Figure 2

Healthy control (HC) and rheumatoid arthritis (RA) tolerogenic dendritic cells (tolDCs) have an anti-inflammatory phenotpye. (A) Mean cytokine production by tolDCs and mature DCs (matDCs) in response to CD40 activation. At 24 h after maturation with 1 µg/ml monophosphoryl lipid A (MPLA) in the presence or absence of dexamethasone (Dex) and vitamin D3 (VitD3), DCs were washed, and 105 tolDCs or matDCs cocultured at a 1:1 ratio with the J588L-CD40L-expressing cell line in the absence of Dex and VitD3 for a further 24 h. Supernatants were harvested for cytokine analysis by ELISA. Data are expressed as the mean±SEM for at least four HCs and seven RA-independent donors. (B) Representative RA donor DC-derived cytokine production when unloaded (UL) or loaded with 10 µg/ml human cartilage glycoprotein 39 (HCgp39) as a model antigen, during maturation with 1 µg/ml MPLA. Unloaded and antigen-loaded DCs were activated via CD40 as described in (A). Data are representative of four independent donors. Cytokine production was determined by ELISA. <d, below limits of detection. Detection limit for IL-12 is 30 pg/ml. *p≤0.05, **p≤0.01, ***p≤0.001 (Mann–Whitney U test). TNF, tumour necrosis factor.