Tab. 7.
HPLC Gradient program for impurities analysis.
| Time (min) | Flow rate (ml/min) | % Mobile Phase A | % Mobile Phase B | Gradient Curve |
|---|---|---|---|---|
| 0–25 | 1.0 | 100 | 0 | Isocratic |
| 25–60 | 1.0 | 100→20 | 0→80 | Linear |
| 60–65 | 1.0 | 20→100 | 80→20 | Linear |
| 65–75 | 1.0 | 100 | 0 | Equilibration |
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