Tab. 8.
HPLC Gradient Program for assay analysis.
| Time (min) | Flow rate (ml/min) | % Mobile Phase A | % Mobile Phase B | Gradient Curve |
|---|---|---|---|---|
| 0–25 | 1.0 | 95→40 | 5→60 | Linear |
| 25–26 | 1.0 | 40→95 | 60→5 | Linear |
| 26–35 | 1.0 | 95 | 5 | Equilibration |
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