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B220−CD11b− cells can differentiate into B cells with the support of OP9 stromal cells. ONP cells were sorted and cultured with SCF, FL and IL-3 for 3 days, phenotype analysis showed <5% cells differentiated into B220−CD11b− cells (A). When sorted B220−CD11b− cells separately and cultured with OP9 stromal cells for another 9 days, over 90% of these cells differentiated into B cells (B).
