Table 1.
Kinetic parameters of fumarases from various sources.
| Substrate/Source | Fumarate | L-Malate | ||||
|---|---|---|---|---|---|---|
| Km (mM) | Vmax (μmol product/min/mg enzyme) | kcat/Km (/mM/s) | Km (mM) | Vmax (μmol product/min/mg enzyme) | kcat/Km (/mM/s) | |
|
Pelotomaculum thermopropionicum (MmcBC) [7] |
0.43 | 201 | 509 | 0.59 | 23.7 | 42.7 |
| Thermus thermophilus AS15-1 (pH7.5) [21] | 1.0 | 1340 | NR | NR | NR | NR |
| Bacterium MPOB (DSM 10017) [24] | 0.25 | NR | 690 | 2.38 | NR | 540 |
|
Pyrococcus furiosus (Pf fumarase) [25] |
0.34 | 1376 | 3200 | 0.41 | 1892 | 3700 |
| Marine samples (FumF, this work) |
0.48 | 827 | 1900 | 3.15 | 282 | 100 |
NR, not reports.