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. 2010 Nov 23;17(1):89. doi: 10.1186/1423-0127-17-89

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Copyright ©2010 Li et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kinetic analysis of NIS mutants. HepG2 cells were transfected with pcDNA3.1 (mock), wild-type (wt), or mutated NIS DNAs. After 24 h, initial rates (4 min time points) of iodide uptake were determined at the indicated concentrations of iodide. Calculated curves were generated using the equation v = (Vmax × [I])/(Km + [I]) + 0.0156 × [I] + 2.4588. The terms 0.0156 × [I] + 2.4588 correspond to background adjusted by least squares to the data obtained with non-transfected cells. Values are mean ± standard error of triplicate assays.