Table 1.

Kinetic analysis of human NIS mutants.

NIS mutants	Vmaxa	Kma
Wild type	7.94 ± 0.2	81.35 ± 8.89
R82A	2.46 ± 0.31***	32.87 ± 5.58**
K86A	5.49 ± 0.61**	68.97 ± 14.08
H226A	2.02 ± 0.48***	71.37 ± 12.43
R228A	4.67 ± 0.45***	91.8 ± 7.2
R239A	2.42 ± 0.45***	171.23 ± 36.12**
R241A	2.15 ± 0.14***	67.64 ± 14.69
E79A	8.55 ± 0.46	110.42 ± 39.87
D163A	9.46 ± 3.1	166.51 ± 37.9*
D233A	8.37 ± 1.6	207.49 ± 72.65*
D237A	7.93 ± 0.52	133.46 ± 43.93*
D311A	6.37 ± 0.69	59.89 ± 16.84
D322A	9.45 ± 1.11	496.6 ± 67.35***
D331A	3.98 ± 0.72***	91.27 ± 15.32

^a Values are mean ± standard error of triplicate assays.

*p < 0.05, **p < 0.01, ***p < 0.001, compared with wild type.