Table 2. Combined summary of transgenic RNAi injections.

Constructs	Methylation	# Embryos	TG	CP	TG Frequency	CP Frequency	Penetrance	CP, not TG
Lentivirus plasmid	No	88	16	0	0.18	0	NA	0
empty
U6; GFP
Lentivirus plasmids	No	248	8	0*	0.03	0*	0*	0
nt1266, nt1894
U6; GFP
Lentivirus plasmid	No	55	26	5*	0.47	0.09	0.15	1
nt622
U6; GFP
SB	Yes	56	32	0	0.57	0	0	0
nt622 scrambled
CpG-free H1; GFP
SB	Yes	79	54	0	0.68	0	0	0
nt622
CpG-free H1; GFP
SB	No	32	4	0	0.13	0	0	0
Empty
U6; GFP
SB	No	108	5	4	0.05	0.04	0.8	0
nt1266, nt1894
U6; GFP
SB	No	57	4	3*	0.07	0.05	0.50*	1
nt1266
U6; no GFP
SB	Yes	73	12	2	0.16$	0.03	0.17$	0
nt1266
U6; no GFP
PB	No	59	10	1	0.17	0.02	0.10	0
nt622
U6; no GFP
No PBase control
PB; nt622	No	112	52	12	0.46	0.11	0.23	0
nt622
U6; no GFP
PBase
PB	No	20	2	1	0.10	0.05	0.50	0
nt622
U6; no GFP
4-5X PBase

*Denotes one or more transgenic embryos showing early embryonic growth arrest prior to palate fusion.

^$Three of 24 resorptions were transgenic.

TG, transgenic embryos; CP, cleft palate; Penetrance, number of transgenic embryos with CP.