Table 1. Comparative analysis of the expression of activating and inhibitory cell surface markers on primary and immortalized ECs.
| Primarymacrovascular | Immortalized | Immortalizedmicrovascular | ExpressionPattern | |
| ECs | HUVEC | EA.hy926 | HMEC | HUVEC/EA.hy926/HMEC |
| Marker | % (mean fluorescence intensity) | ++ >70%+ >20%− >10% | ||
| CD31 | 89±4 (89) | 96±7 (96) | 97± (92) | ++/++/++ |
| CD105 | 97±6 (97) | 99±0.1 (99) | 99±2 (98) | ++/++/++ |
| ULBP1 | 0±1 (66) | 0±0 (46) | 4±1 (43) | −/−/− |
| ULBP2 | 48±7 (59) | 96±2 (63) | 98±1 (131) | +/++/++ |
| ULBP3 | 6±3 (52) | 58±11 (52) | 51±11 (62) | −/+/+ |
| MIC-A | 73±6 (107) | 21±4 (46) | 8±3 (116) | ++/+/− |
| MIC-B | 3±1 (47) | 2±1 (25) | 36±10 (37) | −/−/+ |
| HLA-E | 1±0 (11) | 86±3 (162) | 83±4 (201) | −/++/++ |
| W6/32 | 97±2 (48) | 98±9 (75) | 95±7 (67) | ++/++/++ |
| cmHsp70.1 | 6±4 (12) | 58±5 (69) | 48±6 (54) | −/+/+ |
| SPA810 | 2± (51) | 1± (91) | 2± (44) | −/−/− |
| CD54 | 5±2 (88) | 13±2 (46) | 21±3 (49) | −/+/+ |