Figure 5. Ex vivo induction of SHR and AID.

A. Purified peripheral blood B cells were stimulated with CpG, CD40 ligand, and/or anti-Ig, in the presence of IL2 and IL10 for 3 days, or coculture with anti-CD3 and anti-CD28 activated CD4 T cells for 3 days. B. B cells were activated by anti-CD3 and anti-CD28 activated CD4 T cells through direct coculture, transwell coculture, or activated with CpG for 3 days. Expression of select representative SHR genes were analyzed in activated B cells and activated T cells as a control (B cells and T cells were repurified when T/B direct coculture was the mode of activation) by real-time RT-PCR.