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. 2010 Dec 16;5(12):e15230. doi: 10.1371/journal.pone.0015230

Figure 2. Effects of Aβ oligomers on neuronal m-HKI and ATP levels.

Figure 2

Panel A: Aβ oligomers triggers the release of m-HKI from mitochondria. Western immunoblot analysis of HKI levels in mitochondrial fractions from cultured cortical neurons exposed to 500 nM Aβ oligomers (ADDLs) (D) for 24 hours. Cytochrome c oxidase was used as a loading control. Bars correspond to means ± SE from at least three independent experiments carried out in duplicate. Panel B: Time-dependent toxicity of ADDLs (white bars) or Aβ monomers (gray bars) to mature hippocampal neurons measured using the MTT reduction assay. Values represent the means ± SD of the relative absorbance measured using 6 wells for each experimental condition for three independent experiments. (*) indicates a statistically significant (p<0.005) difference relative to control cultures. Panel C: Aβ oligomers decrease ATP levels in mature cortical neurons. Cultures were exposed to vehicle or 500 nM ADDLs for 12 hours. Values represent means ± SD of the relative levels measured in five independent experiments.