Fig. 3.

GH cell network disruption precludes the sexually dimorphic trait of GHRH calcium responses in GH-eGFP cells. (A and B) Asynchronous calcium responses to GHRH (10 nM) in GH-eGFP cells enzymatically isolated from male (A) and female (B) pituitary glands and dispersed onto coverslips. (C and D) Asynchronous calcium responses to GHRH (10 nM) in single GH-eGFP cells in situ in male (C) and female (D) GH-GFP x GHRH-M2 mice. In both cases, the correlation (R) value after application of GHRH was no different from that observed under control conditions.