Fig. 4.

GHRH calcium responses in the GH cell network are amenable to sexual hormone manipulation in adulthood. (A) Proportion of GHRH-responsive cells in sham-operated and gonadectomized male (Left) and female (Right) mice, treated with and without hormone replacement. Data are mean ± SD. ***P < 0.001; *P < 0.05; ns, nonsignificant. (B and C) Representative calcium traces of GH-GFP cell responses to GHRH in pituitary slices obtained from gonadectomized male (B) and female (C) mice. (D and E) Representative calcium traces of GH-GFP cell responses to GHRH (10 nM) in pituitary slices obtained from a castrated male supplemented with testosterone (D) and an ovariectomized female supplemented with ethinyl estradiol (E).