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Effect of lactacystin on the expression of GCase and net enzymatic activity in control and types 1 and 3 GD fibroblasts. (A) Western blot analysis of GCase in normal and GD fibroblasts. Lac, lactacystin-treated samples; UT, untreated samples. (B) Quantification of GCase protein by densitometric analysis of Western blots. (C) GCase catalytic in normal and types 1 and 3 GD fibroblasts with (Lac) and without (UT) lactacystin (Lac) in the culture medium.
