Fig. 4.

She4p directly interacts with a highly conserved region of the myosin motor domain. (A) Fragments of yeast Myo4p were expressed as GST fusion peptides for pull-down assays. Fragments that bind to She4p are labeled with + and those that fail to bind are labeled with − at the right. (B) SDS-PAGE analysis of pull-down experiments; the fragment containing residues 561–587 of Myo4p can stably bind to purified She4p (Lower). GST fusion peptides were indicated by ● and their degradation products by *. (C) Primary structure comparison of the She4p binding sites from the five S. cerevisiae myosins. Corresponding residue numbers are indicated to the left and right. The predicted secondary structure is shown above the alignment. Identical residues are indicated in yellow, similar ones in green.