Fig. 5.

STAT3-K140me2 binds to the SOCS3 promoter selectively. A ChIP assay was performed with chromatin from IL-6–treated or untreated A4 cells expressing wild-type STAT3, using antibodies against total STAT3, S727-phosphoryl-STAT3, or STAT3-K140me2. The immunoprecipitated DNA was amplified by qPCR, using primers specific for SOCS3, CDCA1, and CD14. Values (percent of the inputs) are the mean ± SD of triplicates performed on three separate chromatin preparations. Data were analyzed using origin75 software and compared by using the unpaired Student's t test. All of the values obtained with each antibody were corrected for background by subtracting the value for untreated cells, which was about 0.1% of the input in each case.