Fig. 2.

Localization of epsin 3 in the wall of the body of the stomach. Immunofluorescence of thick (10 μm) (A and B) and semithin (0.5 μm) (C–F) frozen sections. (A) Epsin 3 is selectively concentrated in parietal cells (arrows) and also present, but at lower concentration, in the mucous-secreting cells that line the stomach lumen (arrowheads). (B) Epsin 3 immunoreactivity is absent from the stomach of epsin 3 KO mice. (C–F) Immunofluorescence images showing that cells positive for epsin 3 are also positive for H/K ATPase immunoreactivity, a specific marker of parietal cells. Note that not all cells within the gastric gland are positive for epsin 3, as seen by DAPI staining of nuclei. (Scale bar, 10 μm.)