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Colocalization of epsin 1 and 3 in parietal cells. (A) Double immunofluorescence. (Scale bar, 10 μm.) (B) Colocalization of epsin 1 (5-nm gold) and epsin 3 (10-nm gold) on coated vesicles as assessed by immunogold labeling of ultrathin frozen stomach sections. (Scale bar, 100 nm.)