Nec-1 combined with Z-VAD prevents photoreceptor loss after retinal detachment. (A) TUNEL (green) and DAPI (blue) staining in detached retina treated with vehicle, Nec-1, Z-VAD, or Nec-1 plus Z-VAD on day 3 after retinal detachment. Quantification of TUNEL-positive photoreceptors (B) and ONL thickness ratio (C) on day 3 (vehicle, n = 12; Nec-1, n = 6; Z-VAD, n = 12; Nec-1 plus Z-VAD, n = 12) and day 5 (vehicle, n = 8; Nec-1, n = 6; Z-VAD, n = 8; Nec-1 plus Z-VAD, n = 8) after retinal detachment (*P < 0.05). GCL, ganglion cell layer, INL, inner nuclear layer. (Scale bar, 100 μm.)