Fig. 2.
MybPlt4/Plt4 mice have more prospective ST-HSCs and MPPs. (A) Representative flow cytometric analyses of viable Lin− cells (Upper) and LSK cells (Lower) from bone marrow of mice of the indicated genotypes. The gates used to distinguish prospective LT-HSCs (CD34− Flt3− LSK), ST-HSCs (CD34+ Flt3− LSK), and MPPs (CD34+ Flt3+ LSK) are shown. Percentages of gated cell populations within the parent populations are indicated. (B) Graphs show mean ± SD of the number per femur of total LSKs and LSK subsets. n = 5–6 mice per genotype. ***P < 0.001; ns, non significant.