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. 2010 Nov 29;107(50):21824–21829. doi: 10.1073/pnas.1012071107

Fig. 3.

Fig. 3.

Expression of Ras-GRF1 in MSN subpopulations and its protein levels in dyskinetic animals. Immunofluorescence of Ras-GRF1 (red), EGFP (green), and nuclear labeling with DAPI (blue) of striatonigral neurons (direct pathway) of M4-EGFP mice (A) and striatopallidal neurons (indirect pathway) of A2A-EGFP mice (C). (Scale bars in A and C: 20 μm.) (E) The graph shows the percentage of the total Ras-GRF1–positive neurons that are GFP-positive, indicating that Ras-GRF1 is expressed equally in each subpopulation. As expected, Ras-GRF1 was not expressed either in the direct pathway of M4-EGFP Ras-GRF1–KO mice (B) or in the indirect pathway of A2A EGFP Ras-GRF1–KO mice (D). (F) Protein levels of Ras-GRF1, Ras-GRF2, and pERK1/2 in intact (I) and lesioned (L) striata of WT mice after 9 d of l-dopa treatment were determined by Western blot analysis. p140 Ras-GRF1 (G) and p135 Ras-GRF2 (H) levels were not altered in dopamine-denervated striata after saline or l-dopa treatment, whereas phosphorylation of ERK1/2 is enhanced only in l-dopa–treated striata (I) (one-way ANOVA, #P < 0.01).