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. 2010 Nov 16;107(50):21248–21255. doi: 10.1073/pnas.1015855107

Fig. 1.

Fig. 1.

Bv8 is strongly up-regulated in premetastatic lungs of mice bearing metastatic tumors. (A) Design and results of the microarray study comparing gene expression in lungs from BALB/c naïve mice and mice bearing nonmetastatic tumors 67NR and metastatic 4T1 tumors. Lungs from different experimental groups are color-coded (gray, from naïve mice; blue, from 67NR-bearing mice; orange, from 4T1-bearing mice). Note that the metastatic gene expression profile is clearly separated from both naïve and nonmetastatic profiles. Each profile column represents one individual mouse. (B) Bv8 protein concentrations in the premetastatic lungs of mice bearing various tumors (n = 3 per group). (C) FACS analysis of Cd11b+Gr1+ cells in the premetastatic lungs of BALB/c mice bearing various tumors. Asterisk indicates significant difference relative to the nonmetastatic group. (D) FACS analysis of different cell populations in premetastatic lungs from mice bearing 4T1 tumors 2 wk after tumor inoculation. (E) Bv8 expression in Ly6GLy6C cell subpopulations isolated from lungs of naïve or 4T1 tumor-bearing mice. In D and E, asterisk indicates significant difference relative to naïve group. Graphs present means ± SEM.