Fig. 2.

Increased G-CSF and Bv8 levels are associated with a metastatic phenotype. (A) Plasma levels of various cytokines in the premetastatic phase in mice bearing nonmetastatic or metastatic tumors. In samples from mice with MDA-MB-231 tumors, m indicates mouse, and h is human. (B) Levels of G-CSF in whole-tumor extracts isolated from mice in the premetastatic phase. (C) Numbers of Ly6G+ cells (per millimeter squared) in various organs during the premetastatic phase in mice bearing 4T1 tumors. Asterisk indicates significant difference relative to naïve group; two asterisks indicate significant difference relative to isotype control (ISO) group (C). Data shown are means ± SEM.