Fig. 3.

Mutants with insertions in coexpressed genes exhibit perturbed levels of glucosinolates and glucosinolate metabolites. (A) Accumulation of indicated selected secondary metabolites (nmol/g of fresh tissue weight) in indicated Arabidopsis genotypes without (open bars) or 16 h after inoculation with E. pisi conidiospores (filled bars). Results represent mean ± SD of three independent plants per genotype. Asterisks indicate a significant difference from respective Col-0 sample (*P ≤ 0.05; **P ≤ 0.01), and hash marks indicate a significant difference between inoculated and uninoculated plants of particular genotypes (^#P ≤ 0.05; ^##P ≤ 0.01; Student's t test). Comparable results were obtained in at least three independent experiments. (B) Schematic diagram of indole glucosinolate biosynthesis, showing locations of genes previously identified and unique genes identified in this study. Biosynthetic enzymes are highlighted by boxes; proteins with other activities by ovals. Diagram adapted from ref. 4.