Fig. 2.
Effect of F185 mutations on the adhesive modality of α4β7. (A) Adhesive behavior of α4β7 293T cell transfectants on h-MAdCAM-1/Fc in shear flow. Rolling velocity was measured for all adherent cells and plotted as a distribution at each wall shear stress. (B) Percentage of cells firmly bound to ligand in the indicated divalent ions under the wall shear stress of 1 dyn/cm2. (C) Cell resistance to detachment in shear flow. The total number of cells remaining bound at increasing wall shear stresses was determined as a percent of adherent cells at 1 dyn/cm2. (D) The initial number of bound cells at 1 dyn/cm2 and integrin cell surface expression level. (E) Adhesive behavior of Q324T wedge mutant and F185/Q324T double mutant α4β7 transfectants under the wall shear stress of 1 dyn/cm2. Error bars are ± s.d. (n = 3). *: P < 0.05; **: P < 0.01; and NS, not significant.