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. 2010 Nov 22;107(50):21388–21393. doi: 10.1073/pnas.1015487107

Fig. 5.

Fig. 5.

Effect of F185 mutations on integrin α4β7-mediated bidirectional signaling. (A) The number of rollingly and firmly adherent WT and F185W transfectants under the shear stress of 0.4 dyn/cm2 with or without cotransfection of talin. Error bars are ± s.d. (n = 3). (B) The number of rollingly and firmly adherent WT and F185W 293T transfectants under the shear stress of 0.4 dyn/cm2 pre or poststimulation by ADP or PMA. Error bars are ± s.d. (n = 3). (C) DIC and IRM images of α4β7 CHO-K1 stable transfectants. The images are representatives from one of three independent experiments. Bar, 50 μm. (D) Coimmunoprecipitation of paxillin with integrin α4β7. Total paxillin and β-actin are shown in the lower rows. (E) Quantification of cell spreading area of 293T transfectants expressing α4β7 WT or S988D mutations. Error bars are ± s.d. (n = 50), **: P < 0.01.