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Schematic view of the pull-down method. cDNAs prepared from patients’ RNAs (1) were subjected to hybridization with the biotin-CAG RNA probe (2); and the hybridized cDNAs were recovered by pull-down with avidin-resin (3, 4), followed by elution (5). The recovered cDNAs were examined by the TaqMan SNP Genotyping assay (6). When SNP sites were heterozygous, the SNP typing with the recovered cDNAs plotted the spots distant from the standard curves.
