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The R456V mutant PM H+-ATPase displays decreased affinity for Ca2+ and Tb3+ ions. ATP hydrolytic activity of the wild type and the R456V mutant was measured with varying concentrations of: (A) Ca2+ ions, (B) Tb3+ ions, and (C) Ho3+ ions. The activity without the addition of ions were set to 100% in A, B, and C. ▪, wild type; □, R456V. All experiments are represented as ± S.D.
