Fig. 1.

Overview of the paraffin-embedded ChIP method (PAT-ChIP). (A) Schematic representation of canonical ChIP and PAT-ChIP procedures starting from the spleen of a leukemic mouse. (B) Agarose gel electrophoresis of DNA samples representative of chromatin used as starting material (input) to perform Cells-ChIP and PAT-ChIP. (C and D) Analysis of the amounts of DNA recovered by Cells-ChIP (C) or PAT-ChIP (D), using the indicated antibodies. The amount of DNA is expressed as the ratio between the amount of total immunoprecipitated DNA (bound) and the amount of input DNA (mock: ChIP performed in absence of antibody). Data are expressed as means ± SD.