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. 2010 Dec;12(12):980–992. doi: 10.1593/neo.101146

Figure 2.

Figure 2

Dex and radiation induce cell death of myeloma cell lines by apoptotic pathway. (A) JC-1 fluorescence in myeloma and BMSCs with Dex (5 εM) and/or radiation (6 Gy) treatments at 6 hours after radiation. The data presented are the triplicate averages of the fluorescence ratio ± SD and are representative of two independent experiments. For specific cell lines, *P < .01 versus Dex, ςP < .005 versus IR. ns indicates not significant. (B) Activity of caspase-3 in myeloma (MM.1S, 5TGM1) and BMSCs (HS-5, SR-4987) treated with Dex and/or radiation. The specificity of caspase-3 activation was confirmed with the N-Ac-Asp-Glu-Val-Asp-CHO caspase-3 inhibitor. Values presented are the mean of triplicate values ± SD and are representative of two independent experiments. *P < .001 versus control, ςP < .001 versus Dex or IR, #P < .05 versus control, £P < .001 versus Dex or IR. ns indicates not significant. (C) Western blot analysis of PARP in myeloma cells (8226, ANBL-6, MM.1S) after Dex (5 εM) and/or radiation (6 Gy). For each treatment, top panels show full-length and cleaved PARP fragment (shown by arrows) and bottom panels show tubulin loading control. The blot shown is a representative of three independent blots. Values of densitometric analysis of cleaved PARP (normalized to tubulin) are shown.