Figure 3.

Effect of RalBP1 overexpression on PC3 growth in vitro and metastasis in vivo. (A) Western blots showing Flag-tagged RalBP1 protein level in PC3 cells after stable transfection of RalBP1 transgene used in panels B to D. (B) In vitro cell number as a function of time quantitated with Alamar blue. (C) In vivo subcutaneous tumor growth assessed by weekly tumor volume measurement in five mice per group with two sites per mouse. (D) In vivo metastatic tumor growth assessment using Xenogen after intracardiac inoculation as described in Materials and Methods in seven mice per group.