Table 5.

Sensitization of Stably BCRP1-Expressing BCRP1-Transduced Cells (UKF-NB-3^BCRP1) to Mitoxantrone by Saq, Saq-NO or WK-X-34 (BCRP1 Inhibitor) Concentrations That Did Not Affect Cancer Cell Viability.

	IC50* Mitoxantrone (ng/ml)	Fold Sensitization†
Mitoxantrone	45.87 ± 7.97
Mitoxantrone + WK-X-34 1 µM	0.17 ± 0.04‡	269.8
Mitoxantrone + saq 10 µM	12.54 ± 1.88‡	3.7
Mitoxantrone + saq 5 µM	34.77 ± 10.04	1.3
Mitoxantrone + saq 2.5 µM	39.66 ± 10.44	1.2
Mitoxantrone + saq-NO 10 µM	9.62 ± 3.24‡	4.8
Mitoxantrone + saq-NO 5 µM	19.73 ± 3.64‡	2.3
Mitoxantrone + saq-NO 2.5 µM	37.73 ± 14.21	1.2

Values are mean ± SD from three independent experiments.

^*Concentration that decreases cell viability by 50%.

^†IC₅₀ mitoxantrone/IC₅₀ mitoxantrone + saq, saq-NO, or WK-X-34.

^‡P < .05 relative to mitoxantrone alone.