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. 2010 Dec;12(12):1041–1053. doi: 10.1593/neo.10916

Figure 2.

Figure 2

Forced expression of TFF3 enhances oncogenicity of MCF-7 cells. Anchorage-independent growth was evaluated by colony formation in soft agar (A), foci formation (B), and growth in suspension culture (C) in the medium supplemented with 10% FBS. (D) Western blot analysis of BAX and BCL-2 protein levels in cell lysates of MCF7-Vec and MCF7-TFF3 cells. β-ACTIN was used as a loading control for cell lysates. (E) Colony formation in soft agar: MCF7-Vec and MCF7-TFF3 cells were seeded in 0.35% agar in a 96-well plate and treated with BCL-2 inhibitor YC137 at the indicated concentrations for 10 days. (F) Three-dimensional Matrigel assay: MCF7-Vec and MCF7-TFF3 cells were cultured in 4% Matrigel in 10% FBS medium for 7 days. Bar, 100 µm in A, B, and F. *P < .05. **P < .01.