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. 2010 Dec;12(12):1041–1053. doi: 10.1593/neo.10916

Figure 4.

Figure 4

Neutralization of TFF3 by TFF3 polyclonal antibody reduces viability of mammary carcinoma cells and inhibits xenograft growth. (A) MCF-7 and T47D cells were treated with 500 µg/ml control rabbit IgG or TFF3-pAb in either 0.5% FBS or 10% FBS medium for 3 days. Cell viability was measured by MTT assay as described in Materials and Methods. (B) Apoptotic cell death was assessed by fluorescent microscopic analysis of cell nuclear staining patterns with Hoechst 33258 dye after treatment for 24 hours. (C) Three-dimensional Matrigel: MCF-7 cells and T47D cells were cultured in 4% Matrigel in 10% FBS medium containing 500 µg/ml nonspecific rabbit IgG or TFF3-pAb for 7 days. MCF-7 xenografts in immunodeficient mice were treated with either control rabbit IgG or TFF3-pAb for 14 days. (D) Tumor growth of TFF3-pAb-treated mice and control rabbit IgG-treated mice. (E) Histologic staining of the tumors with hematoxylin and eosin. Arrow A indicates inflammatory granuloma; arrow B, necrotic tissue within the tumor; and arrow C, residual tumor. Bar, 100 µm in C. *P < .05. **P < .01.