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. 2010 Dec;12(12):1041–1053. doi: 10.1593/neo.10916

Figure 6.

Figure 6

Forced expression of TFF3 reduces anti-estrogen sensitivity in mammary carcinoma cells. (A, B) Colony formation in soft agar: MCF7-Vec and MCF7-TFF3 cells were seeded in 0.35% agar in a 96-well plate and treated with tamoxifen (TAM) or fulvestrant (Fulv) in 10% FBS at the concentrations indicated for 10 days. (C) Three-dimensional Matrigel: MCF7-Vec and MCF7-TFF3 cells were seeded in 4% Matrigel in 10% FBS medium and treated with vehicle (Veh) or 1 µM tamoxifen or 10 nM fulvestrant for 7 days. (D) Colony formation in soft agar: MCF7-Vec and MCF7-TFF3 cells were seeded in 0.35% agar and treated with BCL-2 inhibitor YC137 at 1 or 5 µM alone or in combination with 1 µM tamoxifen or 10 nM fulvestrant for 10 days. *P < .05, **P < .01, ***P < .001 compared with vector control group for the same treatment.